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Testing & Quality

What “Not Detected” Means on a Laboratory Report

“Not detected” on a laboratory report means the substance wasn’t found above the lowest level the method can reliably detect. It doesn’t mean the substance is completely absent. Whether a “not detected” result is reassuring depends on that detection limit: it should be stated and sit well below the relevant legal limit. For microbiology, “not detected” refers to a stated sample size, such as 25 g.

Updated 4 October 2026Part of Shilajit Laboratory Testing

Key facts
  • “Not detected” means below the detection limit.
  • It isn’t proof of zero.
  • The detection limit must be stated and low enough.
  • For microbes, it applies to a stated sample size.

What the phrase means

Every laboratory method has a floor: a level below which it can’t reliably tell whether something is present. “Not detected”, often written “ND” or “n.d.”, means the result fell below that floor. The substance might be absent, or present at a level too low to measure. Neither possibility can be distinguished by that method.

“Not detected” in different tests

How to read ND by test type
TestWhat ND meansWhat to check
MetalsBelow the method’s limitLimit vs GB maximum[1]
PesticidesEach listed pesticide below its limitWhich pesticides were screened
MycotoxinsBelow the method’s limitWhich toxins and limits
PathogensNot found in the tested portionPortion size, such as 25 g

When ND is reassuring

An ND result is meaningful when the detection limit is stated and sits well below the legal or reference limit. For mercury, with a GB supplement limit of 0.1 mg/kg,[1] an ND with a limit of 0.01 mg/kg tells you the level is at most a tenth of the maximum. An ND with a limit of 0.2 mg/kg tells you almost nothing, because the product could still exceed the legal limit.

When the limit is missing

A report that says “ND” without a detection limit can’t be judged. That isn’t necessarily a sign of a bad product, but it is a gap. Ask the brand or laboratory for the limits; see what detection limits mean.

Pesticide screens and ND

A pesticide screen may test hundreds of substances. “No pesticides detected” means none of the substances on that list was found above its limit. Substances not on the list weren’t tested. A good report includes or links to the list.

Microbiology and ND

For pathogens such as Salmonella, results are usually “not detected in 25 g” or “absent in 10 g”. A larger tested portion gives more confidence. For counts, “< 10 cfu/g” is the method’s lower reporting limit; see microbiological testing.

Why “zero” claims are misleading

Marketing sometimes turns ND results into “zero heavy metals” or “metal-free”. That overstates what a laboratory can show, and shilajit’s rock origin makes trace metals likely; see “pure” and “zero metals” claims.

ND and daily exposure

For long-term exposure estimates, some people treat an ND result as equal to its detection limit, a cautious worst case. Multiply that limit by your daily serving in grams to get a maximum daily intake. This conservative approach is sensible for metals that accumulate; see long-term use.

What to ask

  1. What was the detection limit?For each ND result.
  2. How does it compare with the legal limit?
  3. Which substances were screened?For pesticides and mycotoxins.
  4. What portion was tested?For pathogens.

Examples in practice

Two ND results compared (illustrative)

Report A: Mercury ND (LOD 0.005 mg/kg). The level is at most 5% of the 0.1 mg/kg GB limit. Reassuring.

Report B: Mercury ND (no LOD stated). It could be anywhere below the method’s unknown limit. Ask before relying on it.

Putting it into practice

Every time you see ND on a report, look for the detection limit and compare it with the GB limit. For pesticides, check the list of substances; for pathogens, the portion tested. If anything is missing, ask the brand.

Why ND results are common

Modern methods can detect very low levels, but many contaminants are absent or present only in tiny amounts in a well-processed product. ND results are therefore common for pesticides, mycotoxins and pathogens on good reports. For metals in shilajit, numerical results are more common, because trace metals from the source rock are usually present at measurable levels.

In short

ND is only as informative as the limit behind it. A clear limit, well below the legal maximum, turns ND into genuinely reassuring evidence. When the limit is missing or high, ND tells you very little, so ask before you rely on it.

A habit worth forming

Make it a habit to look for the detection limit every time you see ND. Within a few reports, you will spot immediately which ND results are genuinely reassuring and which leave the question open, and you will know exactly what to ask the brand. It quickly becomes second nature.

The bottom line

“Not detected” means below the method’s detection limit, not zero. It is reassuring when the limit is stated and well below the legal maximum, when screens list what they covered, and when microbiology states the portion tested.

Frequently asked questions

What does “not detected” mean?

That the substance wasn’t found above the method’s detection limit.

Does ND mean zero?

No. The substance may be present below the detection limit.

When is an ND result reassuring?

When the detection limit is stated and well below the legal limit.[1]

What does “ND” on a pesticide screen mean?

None of the listed pesticides was found above its limit.

What does “not detected in 25 g” mean?

The organism wasn’t found in a 25 g test portion.

Can a product with ND results claim “zero metals”?

That overstates what testing can show.

How do I use ND results for exposure?

Treat the result as its detection limit for a cautious worst case.

What if no detection limit is given?

Ask the brand or laboratory for it.

Sources & further reading

Primary research, regulators and standards bodies are cited first. A source being listed here does not mean it supports every claim made by sellers; it supports the specific statement it is attached to.

  1. European Commission (UK version on legislation.gov.uk). Commission Regulation (EC) No 629/2008 amending Regulation (EC) No 1881/2006 setting maximum levels for certain contaminants in foodstuffs. legislation.gov.uk, 2008. View source (opens in a new tab) [UK legislation (retained)]